胃癌相关易感基因W41的原位表达分析

Expressive analysis of human gastric cancer-associated susceptible gene in Situ

目的:进一步分析人胃癌相关易感基因W41在胃癌组织的表达情况。方法:利用循环引物介导原位标记(C-PR INS)技术,检测胃癌标本的W41 mRNA表达,并采用生物素连接辣根过氧化物酶标记,DAB显色法,以提高石蜡切片检测mRNA表达的敏感性。用Im age-Pro P lus图象分析软件计算每个细胞胞浆中阳性染色信号的单位面积光密度值(IOD),每例标本IOD的平均值代表W41表达水平。结果:W41在胃癌、有肠化癌旁组织、无肠化癌旁组织和正常胃黏膜中表达结果经F检验有显著性差异(F=8.810,P=0.001)。组间比较结果示胃癌组织表达高于正常组织(P<0.05),癌旁肠化组织和无癌旁肠化组织的表达亦高于正常组织(P<0.05),而癌与癌旁肠化组织和无癌旁肠化组织之间的表达没有差异(P>0.05)。W41在腺癌和印戒细胞癌的表达经t检验无显著性差异(t=1.304,P=0.029)。结论:W41在胃癌及癌旁组织中表达高于正常胃黏膜,在肠化组织有明显表达,提示W41表达水平与胃黏膜癌变有关;C-PR INS法在信号强度、操作便利和时间上明显优于经典原位杂交。

Objectlve：To further study the expression of the human gastric cancer-associated susceptible gene cDNA W41 in gastric cancer tissues. Methods：C-PRINS technology are applied to detect the expression of W41 in gastric cancer and normal tissues, in the same time bioepiderm with HRP（ horseradish peroxidase） labeling and DAB chromogenic method were employed so as to increase sensibility of detecting W41 mRNA on paraffin section. Image analysis sofeware was used to quanfitate the expression of W41, and mean OD was used as the quantitative index. Results：In Situ C-PRINS showed that W41 expressed significant difference in gastric cancer, intestinal metaplasic （IM）, none-IM paratumor tissues and normal gastric mucosas by F test （ F = 8. 810, P = 0.001 ）. W41 expression existed no difference between tumor and paratumor tissue （P 〉 0.05 ）, while its expression was significantly lower in normal tissue than in tumor and paratumor tissues（ P 〈 0.05 ）. there was no difference between adenocarcinoma and signet-ring cell carcinoma（t = 1. 304, P = 0.029）. Conclusion： W41 presented higher expression in gastric cancer tissue,paratumor tissue than normal tissue, and apparente expression in IM, that suggests W41 is linked to the gastric carcinogenisis. C-PRINS method is superior to the classic hybridization in situ, with the advantages of stronger signal, easy manipulation, and time saving.