透明颤菌血红蛋白基因在苏云金杆菌中的表达

Expression of Vitreoscilla Hemoglobin gene in Bacillus thuringiensis

采用大肠杆菌与枯草杆菌的穿梭质粒pMK4作为载体,构建了含有果聚糖酶基因(sacB)启动子和vgb基因的重组质粒pMK-SV.通过原生质体和电转化,将pMK-SV转入苏云金杆菌中,经影印筛选得到转化子Bt4.对Bt4诱导表达,与原菌株相比Bt4的生长量增加了20.2%.

For efficient expression of vgb gene in Bacillus thuringiensis（Bt） , pMK-SV was constructed using pMK4, a shuttle vector between Gram negative and positive, in which vgb gene including sacB promoter was inserted, pMK-SV is transformated into Bt by the method of protoplasm and electroporation. The recombinants were obtained by blotting method. The density of recomhinants increases 20.2 % compared with original stain（HBF-1 ） after sugar inducing.