真菌来源的乙酰胆碱酯酶抑制剂F01-2076A的研究

Study on F01-2076A,a Acetylcholinesterase Inhibitor from Fungus

利用自建的乙酰胆碱酯酶抑制剂的高通量筛选模型,从大量真菌中筛选到1株阳性菌株F01-2076.该菌株的发酵液经有机溶剂提取、硅胶柱层析及HPLC ODS反相柱层析分离,得到1个活性化合物F01-2076A,该化合物对乙酰胆碱酯酶的IC50为23.4μmol/L.通过对该化合物的紫外、质谱、核磁等理化分析,得知该化合物与已知化合物Altenusin结构相同.

A high throughput screening method was established for screening AchE inhibitor from fungus. As a result, an active strain, F01-2076 was isolated. The culture broth of it was processed by solvent extraction, SiO2 column chromatography and ODS HPLC purification , then an active compound named F01-2076A was got. The compound showed inhibitory activity against Ache of ICs0 23.4 μmol/L. By means of physicalchemical properties, ^13C-NMR and 1H-NMR analysis, the structure of this compound was identical with the Altenusin. It was reported as myosin light chain kinase inhibitor and sphingomyelinase inhibitor. The compound is discovered as a Ache inhibitor for the first time.