短期冻存对大鼠骨髓基质干细胞的影响

The effect of short-term cryoprotection on rat bone marrow stromal cells

目的探讨不同冻存条件对大鼠骨髓基质干细胞(BMSCs)复苏后存活、增殖及向肝细胞分化能力的影响。方法比较用含不同浓度血清的冻存液冻存不同时间的BMSCs复苏后的存活率与贴壁率、增殖、向肝细胞分化能力。结果用血清浓度为90%的冻存液经不同时间冻存后的BMSCs复苏后的存活率、贴壁率最高,且其增殖及向肝细胞分化能力与正常对照组相比差异无统计学意义;而血清浓度为50%及20%时BMSCs复苏后的存活率、贴壁率较低,增殖及向肝细胞分化能力下降,与正常对照组相比差异有统计学意义。结论高浓度血清冻存液短期冻存对BMSCs的存活、增殖、分化能力影响较小,细胞复苏后可用于进一步研究。

Objective To investigate the effect of different short-term cryoprotecting methods on the survival, proliferationa and differentiation into hepatocyte of rat bone marrow stromal cells. Methods The survival rate,adherence rate, proliferation and differentiation into hepatocyte of cells resuscitated from. cryoprotection with cryoprotectant of different concentration of blood serum were measured. Results The survival, adherence rate, proliferation and differentiation to hepatocyte of BKSCs with 90% of blood serum in the cryoprotecant were those with 50% or 20% higher than those with 50% or 20% of blood serum in the cryoprotectant. Conclusion The rat bone marrow stromal cells resuscitate from cryoprotection with cryoprotectant containing high concentration of blood senma can be used in further study.