固定化乳糖酶水解乳清粉合成低聚半乳糖的研究

Investigated synthesis of galato-oligosaccharide from whey mist by immobilized lactase

以脱盐乳清粉为原料,研究了用固定化乳糖酶水解乳清粉合成GOS的工艺条件。试验结果表明,反应时间、乳清粉浓度、温度、加酶量及pH对GOS的合成均能产生不同程度的影响,其中乳清粉浓度和反应时间影响较大,pH影响较小。通过正交试验优化出的乳糖酶水解乳清粉催化合成GOS的最佳工艺条件为:反应体系的pH5.0、乳清粉浓度50%、乳糖酶用量40U/g、温度55℃、反应时间为36h。在此条件下GOS的合成率为29.78%。

The optimum conditions of production of galacto-oligosaccharide were investigated using Immobilized lactase acts on desalUng whey mist, and thin-layer chromatography estimates the quantity of gaiactooligosaccharide. The result showed as follow： The primary factors of this reaction were desalting whey mist concentration and time, while the lest one was pH, The synthesis of galacto-oligosaccharide amount was 29.78%, when time was 36h, temperature was 55℃, whey mist concentration was 50%, pH was 5.0, and the enzyme amount was 40U/g.