醛固酮合成酶基因多态性及生活习惯与急性脑梗死易感性的关系

The relationship of aldosterone synthase gene polymorphism, dietary habits and susceptibility to acute cerebral infarction

目的研究醛固酮合成酶(CYP11B2)基因-344T/C多态性及烟酒茶嗜好相互作用与急性脑梗死(ACI)易感性的关系。方法采用病例对照研究方法,调查研究对象的生活习惯,采用聚合酶链反应限制性内切酶长度多态性(PCR-RFLP)方法检测CYP11B2的基因型。结果病例组CYP11B2基因TC和CC基因型的频率显著高于对照组,分别为38.12%和9.97%;相对于TT基因型,暴露于TC和CC基因型人群的OR值分别为1.72和1.88。病例组C等位基因的频率也显著高于对照组,为29.03%;相对于T等位基因,C等位基因的OR值为1.57。此外研究还显示CYP11B2基因TC或CC基因型与吸烟相互作用可增加ACI的易感性,而该基因型与饮酒、饮茶无显著相互作用。结论当个体携带TC或CC基因型时,ACI的易感性增加,该两种基因型与吸烟相互作用,可增加ACI的易感性。

Objective To investigate the relationship between aldosterone synthase （ CYP11 B2） gene-344T/C polymorphism, dietary habits and the risk of acute cerebral infarction （ACI）. Methods The dietary habits data were collected by case-control study. -344T/C polymorphism of CYP11 B2 gene was monitored by polymerize chain reactlon-restriction fragment length polymorphism （PCR-RFLP）. Results The frequency of TC, CC in ACI cases were significantly higher than those in controls （38. 12%, 9. 97% ）. The odds ratios （OR） for those exposed to TC and CC genotypes were 1.72 and 1.88, comparing with that to TT genotype. The frequency of C allele in ACI cases was 29. 03%, significantly higher than that of controls. The OR for those exposed to C allele was 1.57, comparing with that to T allele. In addition, TC or CC genetype interacted with smoking to increase the risk of ACI; No interaction was observed between TC, CC genetype and alcohol drinking or tea intake. Conclusions The CYP11B2-344 TC or cC genetype might increase the risk of ACI development and the genetype might also interact with smoking to increase the susceptibility of ACI.