水痘-带状疱疹病毒糖蛋白E表达载体的构建

Construction of the eukaryotic expression vector containing gene of varicella-zoster virus glycoprotein E

目的构建水痘-带状疱疹病毒(VZV)糖蛋白E的真核表达载体。方法用PCR方法扩增VZV糖蛋白E基因,并将其克隆到真核表达载体pcDNA3.1并用双酶切和测序方法鉴定。结果扩增的目的基因包括了全长的糖蛋白E基因,长度约1.9 kb。并且成功构建了糖蛋白E基因重组表达载体。结论构建的重组表达载体基因序列正确,为进一步研究此蛋白的免疫原性打下了基础。

Objective To construet the eukaryotic expression vector of varicella-zoster virus （VZV） glycoprotein E. Methods VZV glycoprotein E genes were amplified by PCR and cloned into the eukaryotic expressiom vector peDNA3. 1. The construeted vector was identified by double enzyme-digestive method and sequencing. Results Tile amplified gone, which was about 1.9 kb, included entire gene of VZV glycoprotein E. The recombinant vector was identified as pcDNA-VgE. Conclusion The construcled vector containing gene of VZV glycoprotein E can express gtycoprotein E, which lays the fonndation for studying immunogenicity of glycoprotein E.