全脑缺血再灌注损伤早期脑皮质超微结构的变化

Changes of the ultrastructure of global cerebral ischemia and reperfusion damage in rats

目的探讨全脑缺血再灌注损伤大鼠早期脑皮质神经元、胶质细胞和血脑屏障的变化。方法将6只Wistar大鼠随机分为缺血再灌注组(n=3)和假手术对照组(n=3)。制备大鼠全脑缺血再灌注模型。缺血再灌注组于缺血再灌注1h、假手术组于手术后1h取脑,电镜观察皮质超微结构的变化。结果缺血再灌注早期(1h)皮质神经细胞发生不同程度的固缩,胶质细胞肿胀,核内染色质溶解,核膜不清;血管周围足突轻度肿胀,与基膜分离;微管有部分溶解。结论再灌注损伤早期皮质神经元、胶质细胞、细胞骨架和血脑屏障即发生变化。

Objective To investigate the changes of ultrastructure of global cerebral ischemia and reperfusion damage in rats. Methods Six adult male Wistar rats were randomly divided into the ischemia group （n= 3）, and sham injury group （n= 3）. Global cerebral ischemia and reperfusion model were established with Pulsinelli＇s method. Cortical ultrastructure changes were investigated on the first hour after reperfusion. Results In the ischemia group, different degrees of pyconosis neurons, abnormal organell and microtuhe of neurons were observed. In addition, glial cell and microvessels were damaged in ischemia group, but that in sham injury group were not demaged. Conclusion Cortical neurons, neuroglia cells, cytoskeleton and blood-brain barrier changed at early stage of reperfusion damage.