脊髓损伤后白细胞介素-1β、caspase-3的表达和细胞凋亡的相关性

Correlation among apoptosis, expression of interleukin-1β and caspase-3 after spinal cord injury in rats

目的研究大鼠脊髓损伤后白细胞介素-1β(IL-1β)、caspase-3表达和细胞凋亡的规律及三者之间的关系。方法成年SD大鼠随机分为假手术组和损伤组,于伤后1h、8h、24h、72h处死,用免疫组化染色检测IL-1β、caspase-3阳性细胞,TUNEL法标记凋亡细胞。结果假手术组仅表达少量IL-1β和caspase-3阳性细胞;损伤组两者均在8h表达最多,24h减少,72h减少至略多于假手术组水平。TUNEL标记阳性细胞的时间分布特点与IL-1β和caspase-3相似;IL-1β、caspase-3阳性细胞率和细胞凋亡指数三者间存在正相关(P<0·01)。结论大鼠脊髓损伤后,IL-1β和caspase-3表达增强,凋亡细胞大量出现,三者间存在正相关。

Objective To study the characteristic of apoptosis and the expression of interleukin-1β（IL-1β）and caspase-3, as well as the relation among them after spinal cord injury（SCI） in rats. Methods Adult SD rats were divided into two groups randomly： sham group and spinal cord injury group. The rats were killed at 1 h, 8 h, 24 h, and 72h after surgery. The expression of IL-1βand caspase-3 was determined with immunohistochemistry and the apoptosis was labeled by TUNEL. Results There was only a few cells that expressed low level IL-1β and caspase-3 in sham group, but they both enhanced in SCI group within 8 h after operation, and then decreased to the level similar to sham group 72 h after operation. The rates of TUNEL-positive cells were similar with that of IL-1β and caspase-3 after SCI. There was positive correlation among expression of IL-1β and caspase-3 and apoptosis index. Conclusion Expression of IL-1β and caspase-3 increase and a large number of TUNEL-positive cells present after SCI in rats, and there is positive correlation among them.