大鼠脑液压冲击伤caspase-8的表达

Expression of Caspase-8 after Brain Injury from Fluid Percussion in Rats

目的探讨大鼠脑损伤后caspase-8表达情况,为脑损伤的诊断及损伤时间推断提供依据。方法建立大鼠脑液压冲击伤模型。用免疫组化与图像分析技术分别检测伤后15,30min和1,3,6,12h及1,4,7,14d大鼠皮质、丘脑、海马等部位caspase-8的表达。结果发现伤后30min大脑皮质和海马caspase-8开始出现表达,随时间增加其表达亦逐渐增加,伤后3h显著增加,24h达到高峰,4d后逐渐减少,14d基本恢复正常;而丘脑在伤后1h才开始出现阳性表达,伤后6h出现明显表达,24h达到高峰,4d后逐渐减少,14d基本恢复正常。同时发现损伤对侧海马及丘脑出现相应的变化规律。结论caspase-8阳性表达的变化规律可作为脑损伤诊断及损伤时间推测的指标之一。

Objective To provide the evidence of the relationship between brain injury and the time of injury. Methods Rats were contused on brain by fluid percussion, then were killed after injury for 15rain, 30min, 1,3,6,12h, and 1,4,7,14 d respectively. The expression of caspase-8 were detected by immunohistochemical technology on rat brain section and the results were assessed by image analysis system in the cerebral cortex, thalamus, and hippocampus. Results The expression of caspase-8 in cortex and hippocampus could be detected in 30rain after injury, increased significantly in 3h, reached apex in ld after injury, remained 4d before decreased. In addition, the expression of caspase-8 can be detected in lh after injury and reached apex in ld after injury, and remained 4d then reduced. Conclusion It seems that the exvression of casoase-8 should be a useful target for diagnosis of early brain injury.