急性及己中毒的实验病理学研究

Experimental Pathological Study of Acute Intoxication by Chloranthus serratus Roem. et Schalt

目的探讨及己对小鼠毒性作用的病理变化及其毒作用机制。方法应用及己水煎液对小鼠经口急性染毒,进行组织病理学观察血清生化、凝血机能检测。结果及己对小鼠的半数致死量(LD50)为41.12g/kg;染毒小鼠血清ALT和BUN升高;血液血小板计数减少、凝血时间延长;肝、脾、肾器官系数增大;肝、肾、心肌细胞变性、坏死明显,全身诸多器官淤血、出血性改变。结论及己毒作用的主要靶器官或靶组织是肝脏、肾脏、心脏和全身血管。毒作用机制是对线粒体、内质网等膜性结构及体内凝血机制的破坏。

Objective To find out the pathological change and the toxic mechanism of Chloranthus serratus Roem. et Schalt in mice. Methods Mice were intoxicated by oral administration with extracts of Chloranthus serratus Roem. et Schalt followed by pathological, serum biochemical, and coagulation mechanism examination. Results The LD50 in mice was 41.12g/kg; All poisoned mice serum BUN and ALT increased markedly; Thrombocyte decreased and coagulation time increased; The organ index of liver, spleen and kidneys increased significantly; The cells of liver, kidney and heart were degeneration and necrosis, There were extensive hyperemia and hemorrhage in many organs. Conclusion The experiment suggests that the target organs were liver, kidney, heart and blood vessels; The toxic mechanism was the damage on the mitochondrional, endoplasmic reticulum and coagulation system.