16SrDNA序列分析在嗜尸性蝇类鉴定中的应用

Identification of Sarcosaphagous Flies by Analyses the Sequence of 16S rDNA

目的通过mtDNA上16SrDNA中551bp基因序列分析,解决依据COI和COII上278bp和635bp基因序列难以鉴别丝光绿蝇、铜绿蝇种类的难题,为法医学嗜尸性苍蝇种类的鉴别提供依据。方法随机采集放置在呼和浩特及成都地区室外草地家兔尸体上的铜绿蝇和丝光绿蝇,对其mtDNA上16SrDNA中551bp基因序列进行分析、比对,构建系统发育树。结果通过对嗜尸性蝇类mtDNA上16SrDNA的551bp基因序列分析可以对丝光绿蝇和铜绿蝇进行鉴别。结论16SrDNA上551bp基因序列分析是对嗜尸性蝇类(尤其是铜绿蝇和丝光绿蝇)进行种类鉴定的有效方法,是对依据COⅠ和COⅡ序列分析方法鉴别嗜尸性苍蝇种类的重要补充。

Objective To solve the difficulties of identification of Sarcosaphagous flies such as Lucilia sericata （Meigen） and Lucilia cuprina （Wiedemann） which could not be identified by analyzing the 278 bp and 635 bp regions of the gene encoding for cytochrome oxidase subunit I and II （CO I and CO II ） in mtDNA. Methods Specimens were collected from the corpses of rabbits on the grassland in Huhhot and Chengdu, the sequences of 551 bp region of 16S rDNA of their mtDNA were analyzed, the muhiplealignment program DNAMAN（version 4.0） and MEGA 2.1 sofeware were employed for sequence alignments neighbour-joining tree construction. Results Lucilia seficata （Meigen） and Lucilia cuprina （Wiedemann） were distinguished successfully by sequence analysis of The 551 bp region of the gene of 16S rDNA. Conclusion The 551 bp region of the gene of 16S rDNA of sarcosaphagous flies can be used for identifying them on species level effectively. It is likely to be a successful compliment to identify the sarcosaphagous flies by sequence analysis of COⅠ and COⅡ in mtDNA.