γ-羟基丁酸在急性中毒大鼠体液和组织中的检测及分布

Study on Determination and Distribution of GHB in Biological Fluids and Tissues of Acute Poisoned Rats

目的建立生物检材中γ-羟基丁酸(GHB)的检测方法,研究GHB急性中毒大鼠体内GHB的分布,为GHB中毒的鉴定提供方法和评价依据。方法用GC/MS法检测生物检材中的GHB;以1000mg/kg剂量给大鼠灌胃使其染毒,分别于1h和3h处死,测定体液和组织中GHB的含量。结果测组织中内源性GHB的线性范围是1～20μg/g,R2=0.9974;测组织中外源性GHB的线性范围为100～1500μg/g,R2=0.9958。相对回收率为98%～103%。体内内源性GHB的含量均≤10μg/mL或10μg/g。尿液中GHB含量为最高,其他依次为:胃、血液、肠、肾、肺、脾、心、肝和脑。结论所建方法准确、便捷,适用于GHB中毒的鉴定;尿液是体内检测GHB的最佳检材。

Objective To investigate the distribution of GHB in biological fluids and tissues and provide method and information for detection of GHB in Biological Fluids and Tissues Method The concentrations of GHB in Biological Fluids and Tissues were analyzed by gas chromatography-mass spectrometry; Two groups of rats were fed with 1000 mg＆g of GHB and were killed at 1 hour and 3 hours post dose, respectively. Results GHB concentrations of the administrated groups distributed in a degressive order of urine, stomach, blood, intestine, kidney, lung, spleen, heart, liver and brain. Conclusion Urine was the best specimen for the determination of GHB concentrations in biological fluids and tissues.