巴戟天不同农家类型种质资源的RAPD分析

RAPD Analysis on Germplasm Resources of Different Farm Races of Morinda officinalis

目的：探讨不同农家类型巴戟天在分子水平上的遗传多样性。方法：利用分子生物学技术中随机扩增多态性DNA（RAPD）技术对巴戟天5个不同农家类型的种质资源进行检测。结论：40个随机引物中有14个引物扩增产物具多态性，每个多态性引物平均可扩增出3～5个片段。聚类分析表明，全部材料可分为两大类，其中大叶种聚为一类，小叶种单独为一类。结果：不同农家类型巴戟天种质资源在分子水平上存在明显的遗传差异。

Objective： To explore the genetic diversity of different farm races of Morinda officinalis on molecular level. Methods： The molecular biological technique-random amplified polymorphie DNA （RAPD） were used. Results ： Of the 40-mer arbitary primers, 14 were found to amplify polymorphic products. 3-5 polymorphic bands were amplified by each polymorphic primer on the average. Using UPGMA method all the tested accesions can be clustered into two groups ： 4 accessions of Daye were classified as one group, 1 accession of Xiaoye be another group. Conclusion： There actually existed much genetic diversity on molecular level among the different farm races of Morinda officinalis.