油菜叶片中蛋白酶的分析鉴定

以小油菜离体地上植株为研究对象,在室温(20℃)下观测了小油菜叶片的采后衰老蛋白酶的活性变化,并探讨了部分蛋白酶酶学特性。采用改进了的Gelatin-SDS-PAGE方法,研究表明,随着叶片衰老的进程,蛋白酶总活性增强;部分蛋白酶的最佳前处理温度为20～35℃,最佳反应温度为50℃,在pH8下蛋白酶达到最大活性。

The protease activity changes during the senescence of postharvest rapeseed leaves （Brassia campestris L.） are analyzed at room temperature （20℃）. By improved SDS-getatin- PAGE assay, we have studied some characteristics of protease. The results show that the activity of protease was increasing with rapeseed leaf senescence. The optimal pretreating and incubation temperature for the activity of the protease was 20-35℃ and 50℃. The maximal activity was observed at pH8, some activity could be observed on the gel slices incubated at pH5 or 11.