米曲霉β-半乳糖苷酶催化合成低聚半乳糖的工艺研究

研究了反应时间、乳糖浓度、温度、加酶量、pH对低聚半乳糖合成的影响,结果表明,加酶量、乳糖浓度、反应温度的影响较大。通过正交实验确定的米曲霉β-半乳糖苷酶催化合成低聚半乳糖的最佳工艺条件为:pH6.0、温度55℃、乳糖浓度40%、加酶量40U/g、反应时间32h。在最佳工艺条件下,低聚半乳糖的合成率为32.4%。

The effects of time, initial lactose concentration, temperature, enzyme amount and pH on the synthesis of galacto-oligosaccharide from lactose using β-galactosidase were investigated. The result showed as follow： initial lactose concentration, enzyme amount had strong impacts on the galacto -oligosaccharide yield under the optimum conditions： and temperature reaction and the reached 32.4% temperature was 55℃ , the concentration was40%, pH was enzyme amount was 40U/g. time was 32h, initial lactose 6.0, and the