摘要
Aim:To develop an HPLC method for the determination of azithromycin hydrochloride and its related substances.Method:Dikma ODS column and 40 ℃ of column temperature.The mobile phase: 0.1 mol/L ammonium dihydyogen phosphate solution(adjusted pH to 6.5 by triethylamine)and acetonitrile(70∶30).The flow rate was 1.0 mL/min and UV detective wavelength was 215 nm.Results:Some impurities were obviously separated under the chromatographic system described above.The calibration curves were linear between 10.0～50.0 mg/mL(r=(0.999 8)).The average recovery of adding sample was 99.97%.Precision and inter-day RSD were 0.50%(n=6)and 0.79%(n=6).Conclusion:The method is proved to be quick,simple and sensitive.
Aim：To develop an HPLC method for the determination of azithromycin hydrochloride and its related substances. Method： Dikma ODS column and 40℃ of column temperature. The mobile phase ： 0.1 mol/L ammonium dihydyogen phosphate solution（adjusted pH to 6.5 by triethylamine）and acetonitrile（70： 30）. The flow rate was 1.0 mL/min and UV detective wavelength was 215 nm. Results：Some impurities were obviously separated under the chromatographic system described above. The calibration curves were linear between 10.0- 50.0 mg/mL （r = 0.999 8）. The average recovery of adding sample was 99.97%. Precision and inter-day RSD were 0.50% （ n = 6） and 0.79% （ n = 6）. Conclusion： The method is proved to be quick, simple and sensitive.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
2006年第1期91-92,共2页
Journal of China Pharmaceutical University
关键词
盐酸阿奇霉素
HPLC
有关物质
含量测定
azithromycin hydrochloride
HPLC
related substances
determination of content
