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裂殖酵母生物合成L-苯基乙酰基甲醇的研究 被引量：6

Biotransformation of L-Phenylacetylcarbinol (L-PAC) by Free Cells of Shizosaccharomyces pombe
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摘要 用裂殖酵母作为生物催化剂，催化苯甲醛与丙酮酸合成L苯基乙酰基甲醇（L-PAC），后者为合成L-麻黄素等多种药物的前体，转化反应的最佳温度为28℃，pH6．0，时间16h，苯甲醛用量160-200mmol／L，丙酮酸120～180mmol／L，LPAC产量可达15g／L。 The yeast-mediated biotransformation of benzaldehyde and pyruvic acid to L-Phenylacetylcar binol （L-PAC）, a key intermediate for L-ephedrine synthesis, occurs in citrate buffer（pH6.0） containing 10% free yeast cells aged of 18h at temperature of 28℃. The highest L-PAC concentration of 15 g/L was achieved by adding 150mmol/L benzaldehyde with 180mmol/L pyruvic acid in batches.
作者 宁华中 许激扬 许娟 戴冕
机构地区 中国药科大学生化教研室
出处 《药物生物技术》 CAS CSCD 2006年第1期32-35,共4页 Pharmaceutical Biotechnology
关键词 酵母细胞 L-苯基乙酰基甲醇 生物转化 丙酮酸脱羧酶 L-Phenylacetylcarbinol, Biotransformation, Pyruvatedecarboxylase
分类号 R9 [医药卫生—药学]
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参考文献7

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同被引文献129

  • 1谢雪原,周华,高震,韦萍.多酶偶联反应体系的研究和应用[J].化工进展,2004,23(8):864-868. 被引量：3
  • 2蒋巡天,李继珩,吴梧桐.固定化酵母生产L－间羟基苯基乙酰基甲醇的研究[J].中国药科大学学报,1996,27(11):690-695. 被引量：1
  • 3王艳,周林,姚丽丽.耻垢分枝杆菌产乳酸氧化酶条件的研究[J].食品与发酵工业,2006,32(8):28-31. 被引量：3
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药物生物技术
2006年 第1期

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