参环毛蚓中纤溶活性蛋白酶研究

Study on Thrombolytic Enzymes from Pheretima Aspergillum (E.Perrier)

分离纯化参环毛蚓中纤溶活性蛋白酶,并对其活性及氨基酸序列进行分析。采用凝胶层析等方法进行蛋白质的分离纯化,SDS-PAGE法进行分子质量的测定,纤维平板法测定纤溶活性,氨基酸自动分析仪分析氨基酸组成,并对一个具有纤溶活性的蛋白酶进行了序列分析。分离纯化得到4种酶,其中EQY-4活性较强,其N-端序列为:Gln-Ile-Gly-Gly-Thr-Asn-Ala-Ser-Pro-Gly-Glu-Phe-Pro-Pro-Gln-Leu-Ser-Gln-Thr。与文献报道中赤子爱胜蚓及粉正蚓中分离得到的蛋白酶对比,主要序列相近,但也有不同。

Abstract The purpose is to purify thrombolytic enzymes from Pheretima aspergillum （E. Perrier）, and to analyze their activity and amino acid sequence. Gel chromatography was used to purify proteins, and SDS-PAGE was used to determine molecular weight. Thrombolytic activity of enzymes was determined by fibrin plate. 4 kinds of enzyme EQY-1, EQY-2, EQY-3, EQY-4 were purified, and their amino acid components were analyzed. The amino addic sequence of EQY-4 is Gln-Ile-Gly-Gly-Thr-Asn-Ala -Ser-Pro-Gly Glv-Phe-Pro-Pro-Gln-Leu-Ser-Gln-Thr. Sequence of EQY-4 is close to Lumbricus rut）ellus and Eisenia faetida in references.