维甲酸对正常造血祖细胞集落形成的影响

Effect of All trans Retinoic Acid on the Formation of Normal Hematopoietic Progenitor Cell Colony

用细胞培养方法观察了不同浓度（１０－９～１０－５ｍｏｌ／Ｌ）的全反式维甲酸（ＡＴＲＡ）对正常造血祖细胞集落形成的影响。结果表明，随着浓度的增加，ＡＴＲＡ对红系祖细胞集落形成单位（ＣＦＵ－Ｅ、ＢＦＵ－Ｅ）的抑制作用增加，但在１０－８～１０－６ｍｏｌ／Ｌ内对粒单系祖细胞集落形成单位（ＣＦＵ－ＧＭ）的形成有促进作用，１０－６ｍｏｌ／Ｌ的ＡＴＲＡ对巨核系祖细胞集落形成单位（ＣＦＵ－Ｍｅｇ）生长也有促进作用，提示在急性早幼粒细胞白血病（ＡＰＬ）用ＡＴＲＡ治疗过程中，ＡＴＲＡ参与了其正常造血功能的恢复。

Using cell culture method, we investigated the effects of concentration of all trans retinoic acid (ATRA) (10 -9 ￣10 -5 mol/L) on the formation of hematopoietic progenitor cell in normal human bone marrow. Constant suppression on CFU E and BFU E colony formation and coloring was observed in a dose related fashion from 10 -9 ￣10 -5 mol/L ( n =3). ATRA enhanced the total number of granulocytic/macrophage and megakaryocytic colony between concentration of 10 -8 and 10 -6 mol/L and 10 -6 mol/L, respectively. These results suggest ATRA may play a role in the recovery of hematopoiesis during treatment of acute promyelocytic leukemia with ATRA.