摘要
本研究旨在探讨人乳头瘤病毒(HPV)感染与膀胱肿瘤发生发展之间的关系。用体外基因扩增技术,即聚合酶链反应,使用人乳头瘤病毒16和18型引物序列,对34例膀胱癌新鲜手术切除标本、30例正常膀胱粘膜作对照,进行人乳头瘤病毒基因检测,肿瘤标本HPVDNA总阳性率为41.18%;其中HPV16型阳性率为20.60%,HPV18型阳性率为26.47%,合并感染阳性率为5.88%。30例正常对照标本未检出HPV16、HPV18型DNA序列。研究初步表明,高危类HPV16型。
Technology of DNA amplification polymerase chain reaction (PCR) was used to detect human papillomavirus (HPV) type 16, 18 specific DNA sequences were employed in 34 cases of bladder carcinoma and 30 cases of normal bladder. HPV 16, 18 DNA was found in 14 of the tumors (41.18%); HPV 16 DNA in 7 tumors (20.60%), HPV 18 DNA in 9 tumors (26.47%). HPV DNA was not found in 30 cases of normal bladder. The results show that the close association between bladder cancer and HPV (especially HPV 16 and HPV 18 ) infection.
出处
《南京医科大学学报(自然科学版)》
CAS
CSCD
1996年第5期462-464,共3页
Journal of Nanjing Medical University(Natural Sciences)
关键词
膀胱肿瘤
乳头瘤病毒
聚合酶链反应
bladder neoplasms
human papillomavirus
polymerase chain reaction