摘要
抗三尖杉酯碱的人白血病ＨＬ６０细胞的特性１何琪杨，周卫东，纪林，张鸿卿，何农高，薛绍白２（北京师范大学生物系，北京１００８７５，中国）关键词三尖杉酯碱类；白血病ＨＬ６０；多种抗药性；ｍｄｒ１基因；糖蛋白类；流式细胞术；聚合酶链反应目的：研究人ＨＬ６...
IM: To study
the mechanisms of the resistance to harringtonine (Har) in the HL60
cells. METHODS: Growth inhibition, karyotype analysis, flow
cytometry, Western blotting and polymerase chain reaction. RESULTS:
The Har resistant HL60 cell line, named HR20, showed cross
resistance to homoharringtonine, doxorubicin, daunorubicin,
vincristine, and colchicine. The growth doubling time and the cell
numbers in G 1 phase were increased. The accumulation of cellular
daunoru￣bicin in the resistant cells was obviously reduced, but
distinctly increased by tetrandrine and verapamil. The numbers of
telocentromeric chromosome increased and the chromosomal aberration
more occured in the resistant cells. The resistant cells
overexpressed multidrug resistant mdr 1 gene and P
glycoprotein 150 kDa. CONCLUSION: The Har resistant HL60 cell
strain belonged to a multidrug resistance strain, overexpressing
mdr 1 gene and P glycoprotein.
出处
《中国药理学报》
CSCD
1996年第5期463-467,共5页
Acta Pharmacologica Sinica
关键词
三尖杉酯碱
白血病
HL60
多种抗药性
基因
harringtonines
leukemia HL60
multiple drug resistance
mdr 1 genes
glyco￣proteins
flow cytometry
polymerase chain
reaction
