慢性肝病与相关细胞因子水平的研究

Study on the changes of serum IL-6,IL-8,IL-10 and TNF-α levels in patients with chronic hepatic diseases

目的探讨慢性肝病患者血清白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、白细胞介素-10(IL-10)和肿瘤坏死因子(TNF-α)在肝病发展过程中的含量变化及相互关系。方法用放射免疫分析检测105例慢性肝病患者和32例正常对照组血清IL-6、IL-8、IL-10、TNF-α的水平。结果各组慢性肝病患者血清IL-6、IL-8、TNF-α含量高于正常对照组,有统计学意义(P<0.05);IL-10低于正常对照组,有统计学意义(P<0.01);各项指标以肝硬化患者最为显著;肝病患者各组间细胞因子水平差异有统计学意义(P<0.01)。IL-6、IL-8、IL-10、TNF-α水平的变化与肝病病变程度密切相关。结论细胞因子IL-6、IL-8、IL-10和TNF-α在肝病发展过程中相互作用,联合检测其水平的变化对探讨慢性肝病的诊断、治疗和预后具有重要意义。

Objective To investigate the changes of serum IL-6,IL-8,IL-10 and TNF-α levels in patients with chronic hepatic diseases of various types and stages. Methods Serum IL-6,IL-8,IL-10 and TNF-α levels were measured with RIA in 105 patients with chronic hepatic diseases and 32 controls. Results Serum levels IL-6, IL-8 and TNF-α were significantly higher in patients with chronic hepatic diseases than those in controls（P〈0.05）, but serum IL-10 levels were significantly lower in the patients （P〈0. 01 ）, especially for those patients with liver cirrhosis. There were significantly differences of these cytokines levels among the different types of patients. Serum IL-6,IL-8, and TNF-α levels were positively correlated with the degrees of pathological changes of hepatic disease, but serum IL- 10 levels were negative correlation. Conclusion These cytokines participate in the pathogenesis of chronic hepatic disease. Combined measurements of their serum levels may be of important value for the diagnosis, treatment and outcome prediction of the disease.