大黄素抗肝纤维化的细胞学机制

Cytology mechanism of emodin on anti-hepatic fibrosis.

研究大黄素抗肝纤维化的细胞学机制。分离、培养大鼠肝星状细胞(HSC)并分别以不同剂量的大黄素(大、中、小剂量组分别为(20、10、5)mg/L)干预,测定各组HSC细胞增殖和胶原合成,并测定HSC培养上清液中透明质酸(HA)和层粘连蛋白(LN)含量,结果大黄素干预的HSC增殖、胶原合成、HA及LN合成明显低于正常对照组,且具有剂量依赖关系,结果提示,大黄素对HSC增殖、细胞外基质合成的直接抑制作用可能是其抗肝纤维化的主要作用机制。

To study the cytology mechanism of emodin on anti - hepatic fibrosis. Hepatic stellate cells （HSC） were isolated, cultured and intervened with emodin （low- , medium- and high- dose groups：5, 10,20 mg/L）. The proliferation and collagen synthesis of HSC were detected. The hyaluronic acid （HA） or laminin （LN） of HSC culture medium were analyzed. The results showed that emodin inhibited the proliferation and collagen, HA and LN synthesis of HSC in a dose - dependant manner. The resuits suggested that the direct inhibitory effect of emodin on the proliferation and extracellular matrix synthesis of HSC may be its main mechanism on anti - hepatic fibrosis.