基质金属蛋白酶-9及其抑制物-1在原发性肝癌中的表达及临床意义

The expression of MMP-9 and TIMP-1 in HCC and clinical significance

探讨肝细胞癌(HCC)中MMP-9及TIMP-1mRNA的表达,及其与HCC侵袭、转移等生物学行为的关系。使用RT-PCR法检测20例HCC及癌周组织手术切除标本的MMP-9、TIMP-1mRNA表达。结果显示HCC组的MMP-9、TIMP-1mRNA的相对含量均高于癌周对照组。有包膜浸润的HCC的MMP-9 mRNA表达明显高于无包膜浸润HCC。提示MMP-9、TIMP-1在HCC的浸润和转移中起重要作用。MMP-9 mRNA表达与肝癌细胞包膜浸润密切相关。

To investigate the molecules responsible for the invasion potential of HCC by focusing on matrix metalloproteinase （MMP） in particular, MMP - 9 and the corresponding tissue inhibitor of metalloproteinase （ TIMP - 1 ）. Semi - quantitative Reverse transcription polymerase chain reaction （ sRT - PCR） was used to examine the expression of MMP - 9 and TIMP - 1 mRNA in twenty pairs surgical specimens of HCC and adjacent non - tumoral liver which were obtained from patients undergoing surgical resection. The correlation between expression of MMP- 9 and TIMP- 1 mRNA and clinic opatholigical features was observed. The levels of MMP - 9 mRNA and TIMP mRNA in the tumorous tissues were significantly higher than those in the nontumorous tissues （ P 〈 0.01, P 〈 0.05）. It was found that the expression of MMP- 9 mRNA in HCC with capsular infiltration was significantly higher than in HCC without capsular infiltration. The result here indicated the correlation between expression of MMP - 9 and TIMP- 1 mRNA and the important histopathological characteristics regarding invasion and metastasis. The levels of MMP- 9 mRNA in HCC suggests MMP- 9 closely participates in capsular infihration.