摘要
目的:研究大孔吸附树脂分离富集赤芍中芍药苷的性能。方法:采用HPLC法测定芍药苷的含量;考察大孔吸附树脂对芍药苷的吸附性能以及洗脱参数。结果:D101大孔吸附树脂对提取液中芍药苷的最佳分离条件为:上柱液浓度为2.5mg/mL、流速为3.6BV/h,芍药苷比吸附量达32.2mg/g,20%乙醇洗脱率为91.25%,芍药苷纯度可达73.70%。结论:D101大孔吸附树脂可用于赤芍中芍药苷的分离富集。
Objective：To study the separation effect of D101 macroporous resin for the purification of Paeoniflorin in Radix Paeonia. Methods：HPLC was used to analyze the content of Paeoniflorin, and the adsorption performance and the elution parameters were investigated. Results：The optimal Paeoniflorin was 2.5mg/mL with a flow rate of 3.6BV/h, separation conditions were as follows：the concentration of and 20% alcohol was used as eluant. The adsorption of Paeoniflorin was 32.2mg/g, and the elution ratio of Paeoniflorin was 91.25 % ,and the purity of Paeoniflorin reached 73 70%. Conclusion：D101, resin can be used to refine the Paeoniflorin in the extraction of Radix Paeonia.
出处
《中国实验方剂学杂志》
CAS
2006年第2期25-27,共3页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
大孔吸附树脂
赤芍
芍药苷
吸附
分离
Macroporous resin
Radix Paeoniae Rubra
Paeoniflorin
Adsorption
Separation
