小鼠新分泌蛋白基因mBolA1的克隆与鉴定

Molecular Cloning and Characterization of mBolA1,a Novel Mouse Secreted Protein

采用生物信息学工具预测与实验相结合的方法得到了一个新的小鼠分泌蛋白基因mBolA1。该基因定位于染色体3F2,cDNA全长为730bp,编码137个氨基酸的蛋白,该蛋白含有一个保守的BolA结构域,等电点为9.05。用RT-PCR方法从鼠的混合cDNA库中克隆到mBolA1。Western blot实验表明mBolA1能从瞬转的COS-7细胞中分泌到细胞培养液中。亚细胞定位显示mBolA1定位于细胞浆,且与高尔基体不共定位,提示它是个非经典分泌途径的分泌蛋白。RT-PCR显示mBolA1在组织中广泛表达。它的具体功能有待进一步研究。

Using bioinformatical tools and experimental approaches, a novel secreted protein gene, mouse BoIA1 was obtained . mBolA1 spans 730bp of cDNA and locates on mouse chromosome 3F2, which encodes a protein with 137 amino acids, mBolA1 has a conserved BolA domain and the PI is 9.05. The coding sequence of mBolA1 was cloned by RT-PCR method from cDNA pool composed of mouse tissues cDNA. Western blot showed that mBolAl was secreted out from the transiently transfected COS-7 cells. Subcellular localization showed that mBolA1 protein located in cytoplasm and didn＇t co-localized in Golgi, which indicate that mBolA1 is a unconventional secretion/nonclassical secretory protein. RT-PCR result showed this gene was abundantly expressed in mouse tissues. The particular functions of this gene need further investigation.