活血化瘀注射液I号对大鼠高血压性血-脑屏障破坏的保护作用

Protective Effect of HHI-I Against Phenylephrine-Induced Blood-Brain Barrier Permeability Breakdown in Rats

[目的]探讨活血化瘀注射液I号(HHI-I)对大鼠高血压性血-脑屏障破坏的保护作用。[方法]将大鼠随机分为6组,10只/组:空白对照组(不作任何处理),高血压模型组(股静脉内注射苯肾上腺素后不治疗),低、中、高剂量给药组(静脉内注射1.5%、3.0%、6.0%浓度的HHI-I,2mL/kg,和治疗对照组(以生理盐水代替HHI-I)。各组动物按设计分组处理,60min后股静脉内注射伊文思蓝,再5min后给予苯肾上腺素,30min后处死动物取脑组织标本,测定伊文思蓝(EB)含量。[结果]模型组血-脑通透性明显增加,与正常组比较P<0.05;生理盐水未使增高的通透性降低,而3个剂量的HHI-I均能使通透性明显降低,P均<0.05。[结论]HHI-I对大鼠高血压性血-脑通透性破坏有明显保护作用。

[Objective] To investigate the protective effect of HHI-I against the blood-brain barrier（BBB） permeability breakdown induced by phenylephrine in hypertensive rats. [Methods] Sixty male Wistar rats, weighing about 220 g were randomly divided into 6 groups with IO animals in each. While the blank control group and model group were treated with nothing,three treating groups were received low, moderate and high dose of HHI-I （1.5%, 3% and 6%, 2 mL/kg respectively）. The control group didn＇t receive any treatment but equivalent normal saline. One hour after such intervention all these groups were intravenously injected with the indicator Evans blue, 2%, 4 mg/kg, b.w. Five minutes later the hypertension was induced in the last five groups with phenylephrine, 0.01%, 1.5 mg/kg, b.w. to result in BBB breakdown. Thirty minutes after that all the rats were killed and the cortex of hemisphere was harvested to measure the concentration of Evans blue in it. [Results] The BBB permeability was obviously increased in the rats of model group compared with the blank control（P〈0.05）. No change of BBB was observed in the N. S. control group, in contrast with those in model group, all the three doses of HHI-I could markedly decline the increased BBB permeability induced by phynylephrine. [Conclusion] HHI-I has significant protective effect against BBB permeability breakdown in hypertensive rats.