摘要
目的 探讨肺内源性急性呼吸窘迫综合征(ARDSp)和肺外源性急性呼吸窘迫综合征(ARDSexp)患者细胞因子、糖皮质激素(GCs)度其受体(GR)水平的差异。方法 分别采用酶联免疫吸附实验法(ELISA)。放射性免疫法(RIA)3H标记地塞米松(^3H—DEX)放射性配体结合法检测11例ARDSP患者、10例ARDSexp患者和11例健康志愿者血清中肿瘤坏死因子(TNF-α)、白细胞介素4、10(IL-4、IL0-10)水平.GCs含量,外周血单个核细胞(PBMC)上糖皮质激素受体表达水平。结果 ①ARDSp、ARDSexp患者GR表达水平明显低于正常人(P〈0.01),其GCs明显高于正常人(P〈0.05~0.01)。②ARDSp、ARDSexp患者血清TNF-α、IL-4、IL-10的浓度均明显高于正常人(P〈0,05~0.01)。③与ARDSp组相比.ARDSexp组血清TNF-α、IL-4、IL-10水平明显升高(P〈0.05),GR表这水平明显降低(P〈0.05),而GCs的水平差异无统计学意义(P〉0.05)。结论 ARDSp和ARDSexp组血清TNF-α、IL-4、IL-10水平度GR表达有一定差别。
Objective To explore the differences of cytokines, glucocortieoids(GCs) and glucocorticoid receptor (GR) level between pulmonary acute respiratory distress syndrome(ARDSp) and extrapulmonary ARDS (ARDSexp). Methods Plasma levels of tumor necrosis faetor-α(TN-α),interleukin-4(IL-4), interleukin-10 (IL-10), GCs and the level of GR in peripheral blood mononuclear eells(PBMC) in 11 patients with ARDSp, 10 patients with ARDSexp and 10 healthy volunteers were deteeted by enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA) and radioligand binding assay using ^3H-DEX as ligand. Results ① GR specific binding sites of ARDSp and ARDSexp patients were markedly lower than those of the normal group( P 〈0. 01); and the concentration of their serum GCs was markedly higher than that of the normal group( P〈0.05-0. 01).②The concentrations of serum TNF-α, IL-4, IL-10 in both ARDSp and ARDSexp groups were markedly higher than those of the normal group( P〈0. 05 - 0. 01). ③ Compared to ARDSp group, the levels of serum TNF-α, IL-4, IL-10 in ARDSexp group were markedly higher( P 〈0.05), and GR specific binding sites were markedly decreased( P 〈0.05), the concentrations of GCs in serum of both ARDSp and ARDSexp groups were not remarkably different( P〉0.05). Conclusion The serum levels of TNF-α, IL-4, IL-10 and GR specific binding sites were different between ARDSp group and ARDSexp group.
出处
《临床荟萃》
CAS
北大核心
2006年第4期240-242,共3页
Clinical Focus