摘要
目的 探讨肺内源性急性呼吸窘迫综合征（ARDSp）和肺外源性急性呼吸窘迫综合征（ARDSexp）患者细胞因子、糖皮质激素（GCs）度其受体（GR）水平的差异。方法 分别采用酶联免疫吸附实验法（ELISA）。放射性免疫法（RIA）3H标记地塞米松（^3H—DEX）放射性配体结合法检测11例ARDSP患者、10例ARDSexp患者和11例健康志愿者血清中肿瘤坏死因子（TNF-α）、白细胞介素4、10（IL-4、IL0-10）水平．GCs含量，外周血单个核细胞（PBMC）上糖皮质激素受体表达水平。结果 ①ARDSp、ARDSexp患者GR表达水平明显低于正常人（P〈0．01），其GCs明显高于正常人（P〈0．05～0．01）。②ARDSp、ARDSexp患者血清TNF-α、IL-4、IL-10的浓度均明显高于正常人（P〈0，05～0．01）。③与ARDSp组相比．ARDSexp组血清TNF-α、IL-4、IL-10水平明显升高（P〈0.05），GR表这水平明显降低（P〈0．05），而GCs的水平差异无统计学意义（P〉0．05）。结论 ARDSp和ARDSexp组血清TNF-α、IL-4、IL-10水平度GR表达有一定差别。
Objective To explore the differences of cytokines, glucocortieoids（GCs） and glucocorticoid receptor （GR） level between pulmonary acute respiratory distress syndrome（ARDSp） and extrapulmonary ARDS （ARDSexp）. Methods Plasma levels of tumor necrosis faetor-α（TN-α）,interleukin-4（IL-4）, interleukin-10 （IL-10）, GCs and the level of GR in peripheral blood mononuclear eells（PBMC） in 11 patients with ARDSp, 10 patients with ARDSexp and 10 healthy volunteers were deteeted by enzyme-linked immunosorbent assay （ELISA）, radioimmunoassay （RIA） and radioligand binding assay using ^3H-DEX as ligand. Results ① GR specific binding sites of ARDSp and ARDSexp patients were markedly lower than those of the normal group（ P 〈0. 01）; and the concentration of their serum GCs was markedly higher than that of the normal group（ P〈0.05-0. 01）.②The concentrations of serum TNF-α, IL-4, IL-10 in both ARDSp and ARDSexp groups were markedly higher than those of the normal group（ P〈0. 05 - 0. 01）. ③ Compared to ARDSp group, the levels of serum TNF-α, IL-4, IL-10 in ARDSexp group were markedly higher（ P 〈0.05）, and GR specific binding sites were markedly decreased（ P 〈0.05）, the concentrations of GCs in serum of both ARDSp and ARDSexp groups were not remarkably different（ P〉0.05）. Conclusion The serum levels of TNF-α, IL-4, IL-10 and GR specific binding sites were different between ARDSp group and ARDSexp group.
出处
《临床荟萃》
CAS
北大核心
2006年第4期240-242,共3页
Clinical Focus
