高效液相色谱法测定水中多杀菌素的残留量

Determination of spinosad residues in water by HPLC

建立了一种测定水中多杀菌素残留量的高效液相色谱法。用二氯甲烷提取,选择性地使用固相萃取(SPE)净化,浓缩定容后测定。试验结果表明:多杀菌素在自来水中的平均回收率为90.51%￣96.38%,变异系数为3.59%￣8.72%;在河水中,采用液液分配法处理,平均回收率比在自来水中的平均回收率低,为88.13%￣91.84%,变异系数为3.49%￣7.71%;在河水中,采用液液分配加SPE净化法处理,平均回收率比前两种都低,为79.78%￣81.88%,变异系数为2.51%￣6.35%。多杀菌素在水中最低检出浓度为0.00125mg/L。

An analytical method was developed for determining spinosad residues in water by high performance liquid chromatography （HPLC）. Residues of spinosad in water were extracted with dichloromethane, with an optional sample cleanup using silica solid-phase extraction （SPE）. Extracts were then concentrated to a set volume and analyzed. Recovery of spinosad from tap water was 90.5-96.4%, with a coefficient of variation of 3.59-8.72%. Recovery of spinosad from river water using liquid partitioning was lower than that from tap water, with recovery of 88.1-91.8% and a coefficient of variation of 3.49-7.71%. When using liquid partitioning coupled with SPE cleanup, recovery of spinosad in river water was lower than the previous two methods, with recovery of 79.8-81.9% and a coefficient of variation of 2.51-6.35%. The lower limit of detection of spinosad in water was 0.00125 mg/L.