壳寡糖对肿瘤抑制作用实验研究

Study on Inhabition Effect of Chitlsan Oligosaccharide on Tumour

目的观察壳寡糖对肿瘤抑制作用的影响。方法采用体内注射的方法将壳寡糖注入荷瘤小鼠腹腔及瘤内,观察肿瘤生长情况及MTT比色法观察壳寡糖对肿瘤细胞的体外作用。结果壳寡糖对荷瘤小鼠肿瘤的生长有抑制作用,显微镜观察显示肿瘤组织出现坏死;壳寡糖对肿瘤细胞生长有抑制作用,其抑瘤率与浓度有关,与时间无关,细胞经壳寡糖作用后,透射电镜显示细胞有凋亡趋势。结论壳寡糖可抑制肿瘤生长,同时壳寡糖可能诱导肿瘤细胞凋亡。

Objective To observe the inhibition effect of chitlsan oligosaccharide on tumour. Methods The growth of tumour were observed after chitlsan oligosaccharide was injected into abdominal cavity （ip） or introtumour of mice; And the anti proliferation activities of chitlsan oligosaccharide on cells were evaluated by means of MTT assay. Results Growth of tumour were inhibited by chitlsan oligosaccharide, necrosis was observed in the tumour tissue. Chitlsan oligosaccharide affected the growth of cell. The effect was related to concentration of chitlsan oligosaccharide, but not depending of culture time. The apoptosis of cells could be seen under the transmission electron. Conclusion Chitlsan oligosaccharide could inhibit the growth of tumour and might induce apoptosis of cell.