失血性休克复苏大鼠肺组织中NO、NOS变化及丹参影响

Changes of Pulmonary NO and NOS and Effects of Salvia Miltiorrhiza in Rats Resuscitated after Hemorrhagic Shock

目的观察失血性休克复苏大鼠肺组织中的NO、NOS的变化以及复方丹参注射液的影响,探讨失血性休克复苏大鼠肺损伤的机制及丹参的治疗机制。方法健康SD大鼠30只,随机均分成正常对照组(NC组)、失血性休克复苏组(HS组)和丹参治疗组(SM组)。实验结束时,取全肺测定肺系数,取血和肺组织测定一氧化氮(NO)含量和一氧化氮合酶(NOS)的活性,并对肺组织作光镜病理学检查。结果与HS组相比较,SM组血清和肺组织中的NO含量、NOS活性均显著升高;肺系数降低;肺组织病变明显减轻。结论大鼠失血性休克复苏后,NO的大量释放参与肺损伤过程,丹参对这种肺损伤有良好的保护作用。

Objective To observe effects made by changes of nitric oxide （NO）, nitric oxidesynthase （NOS） and composite salvia miltiorrhizae injection （CSMI） on lungs of hemorrhagic shock resuscitated rats, and to explore the injury mechanism on lungs of hemorrhagic shock resuscitated rats and treating mechanism of salvia miltiorrhizae （SM）. Method To randomly divide on average 30 healthy SD rats into normal control group （Group NC）, hemorrhagic shock group （Group HS） and salvia miltiorrhizae group （Group SM）. When the experiment finishes, select the whole lungs to test the lung coefficients, select blood and lungs to determine the activity of NO and NOS, and make a pathological investigation of the lungs. Result To be compared with group HS, serum content, and NO content and NOS activity in lungs in group SM increase drastically; lung coefficients decrease; pathological changes of lungs apparently weaken. Conclusion After rats hemorrhagically shocking resuscitate, NO emits numerously to participate in the course of lung damnification, and SM plays a good and protective role in lung damnification.