火针对类风湿关节炎模型大鼠血清皮质醇和IL-1β的影响

Influence of Red-hot Needle Puncture on Serum Cortisol and IL-1 β in A Rat Model of Rheumatoid Arthritis

目的探讨火针治疗类风湿性关节炎的作用机理。方法以完全弗氏佐剂关节局部注射造成大鼠类风湿关节炎模型(AA),随机分为正常对照组、模型组、火针组。检测比较火针治疗后各组血清皮质醇、IL1β的含量,及大鼠关节肿胀的变化。结果模型组大鼠血清IL1β含量较正常组显著升高(P<0.05),而血清皮质醇含量较正常组显著降低(P<0.05),关节肿胀度较正常组显著升高,治疗后火针组血清皮质醇升高,IL1β降低,两者分别同模型组比较差异显著(P<0.05),而与正常组对比无差异(P>0.05)。火针组大鼠关节肿胀度亦较模型组显著降低(P<0.05)。结论火针可以降低AA大鼠关节肿胀度,火针对血清皮质醇、IL1β的调节可能是火针治疗类风湿关节炎的内在机制之一,火针降低AA大鼠关节肿胀度亦可能与该调节有关。

Objective To investigate the mechanism of the therapeutic action of red-hot needle puncture on rheumatoid arthritis. Methods A rat model of rheumatoid arthritis（RA） was made by an injection of Freund＇ s adjuvant into the joint. The rats were randomly allocated to normal control, model and red-hot needle puncture groups. Cortisol and IL-1β contents of serum and a change in rat＇ s arthroncus were examined after treatment with red-hot needle puncture and compared between the groups. Results In the model group, IL-1β content of serum was significantly higher （ P 〈 0. 05 ） but cortisol content of serum was significantly lower （ P 〈 0. 05 ） than in the normal group and the degree of arthroncus significantly increased as compared with the normal group. Serum cortisol rose and IL-1β dropped in the red-hot needle puncture group after the treatment, and both were significantly different from those in the model group （ P 〈 0. 05 ） but not from those in the normal group （ P 〉 0. 05 ）. The degree of arthroncus was significantly reduced in the red-hot needle puncture group as compared with the model group （P 〈 0. 05）. Conclusion Red-hot needle punctured can reduce the degree of arthroncus in RA rats. The regulation of serum cortisol and IL-1β by red-hot needle puncture may be one of the intrinsic mechanisms by which it treats rheumatoid arthritis. Red-hot needle puncture reducing the degree of arthroncus in RA rats may also be related to that regulation.