摘要
骨形态发生蛋白(BMPs)是一类调节骨组织发育的生长因子。BMP-2是BMP家族中诱骨活性最强的。在骨组织 工程研究和临床应用中需要大量的BMP-2。因此,研究出一种能够有效地大量生产BMP-2的方法是十分必要的。随着植 物分子生物学的进展,转基因植物被用作一种生物反应器来生产目的蛋白。以gus作为报告基因,研究了重组人bmp-2基 因在烟草中的表达。通过GUS活性检测、半定量PCR和Western blotting分析了根、茎、叶组织中基因表达的水平,结果 显示融合蛋白在根和茎组织中表达量显著高于叶组织。由于根和茎组织中蛋白组成与叶组织相比相对简单,提示其更易于 进行目的蛋白的纯化。
The bone morphogenetic proteins （BMPs） are a family of growth factors that regulate the development of bone. BMP-2 is the most effective in the induction of bone tissue. A large amount of BMP-2 is needed for both bone tissue engineering research and clinical application. Thus, an effective way is necessary to produce sufficient BMP-2 protein. With the advance in plant biotechnology, transgenic plants have been targeted as a bioreactor to produce desired recombinant proteins. Here, the expression of recombinant human bmp-2 gene （rhbmp-2） was studied in tobacco plants using gus as a reporter gene. The difference of expression levels in root, stem and leaf tissues was analyzed by GUS activity assay, semi-quantitive RT-PCR and western blotting.The results indicated that the expression levels of fusion protein in root and stem tissues were significantly higher than those in leaf tissue. For the protein compositions in root and stem tissues were simpler than those in leaf tissue,this suggested that the purification process with root and stem tissues would potentially be easier.
基金
This Work Was Supported by Chinese Academy of Sciences "100 Talented Scholar Program" and Chinese Academy of Sciences "Knowledge Innovation Program" Grants (No.KSCX2-SW-205 KSCW2-SW-218), and also Supported by the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry. The authors also gratefully acknowledge the support of K.C. Wong Education Foundation, Hong Kong.
