尿流式细胞术检测对移植肾急性排斥反应的诊断

Usage of urine flow cytometry in diagnosing the acute renal allograft rejection

目的:探讨尿流式细胞术检测对移植肾急性排斥反应的诊断价值。方法:对63例肾移植术后患者在住院期间出现血肌酐值升高,均行尿流式细胞术检测,结果与临床诊断作对比分析。结果:急性排斥反应患者的尿液细胞数超过10000以上的占97.6%,CD54阳性细胞的占81.8%,CD103阳性细胞占57.6%,HLA-DR阳性细胞占90.9%;在其他患者中,缺少特异性抗原的表达。结论:尿液中HLA-DR、CD54、CD103阳性细胞可作为急性排斥反应的特异标志。尿流式细胞术能区分急性排斥反应与其他因素引起的移植肾功能损伤。尿流式细胞术可作为评价移植肾功能的检测指标。

Objective：To explore the use of urine flow cytometry（UFC） as a noninvasive tool in diagnosing the acute renal allograft rejection. Methods： In this study UFCs were performed in 63 patients at the time of admission to the hospital for the evaluation of risingcreatiniue. UFC analysis were clone blinded to the clinical diagnosis, and results were compared with the discharge diagnosis. Results： The specificity value for the diagnosis of AR was： 90. 9% for the presence of HLA-DR positive cells, 81. 8% for the presence of CD54 and 57.6% for the presence of CD103. The other samples were remarkable for the lack of expression of the antigens studied. Conclusions：These results suggest that UFC may he useful in monitoring renal transplant patients for acute rejection and as a discrimi nator from other causes of renal allograft dysfunction.