粘蛋白7在膀胱癌尿脱落细胞中的表达及意义

Diagnosis value of MUC7 in the detection of bladder transitional cell carcinoma

目的:探讨粘蛋白7(mucin7,MUC7)在膀胱移行细胞癌(TCC)诊断中的意义。方法:采用巢式逆转录聚合酶链反应(nested RT-PCR)方法检测MUC7mRNA在TCC患者尿脱落细胞中的表达。结果:68例TCC患者中有42例尿液MUC7表达阳性,敏感性为62%;MUC7表达与临床分期有关而与病理分级无关。对照组25例中有3例在尿液中MUC7为阳性表达,特异性为88%。结论:用RT-PCR的方法检测尿脱落细胞中的MUC7,具有较高的膀胱癌诊断价值。

Objective：To investigate the diagnosis value of MUC7 in the detection of bladder transitional cell carcinoma. Methods： Expression of MUC7 was detected in exfoliated cells in urine from patients with bladder cancer hy nested reverse transcriptase polymerase chain reaction. Results： Among the 68 patients with bladder transitional cell carcinoma, nested RT-PCR for MUC7 was found positive in 42 （62%）. Based on the tumor stage. MUC7 was positive in 19 of 38（50%） patients with superficial disease （T4- T1 ） and in 23 of 30 （77%） invasive disease patients （T2-T1）. There was a significant difference between superficial and invasive disease, hut no relation between the expression of MUC7 and the tumor grade. And 3 of 25 urine samples of the control group were MUC7 positive, with an overall specificity of 88%. Conclusions：MUC7 is a new reliable urinary tumor marker for bladder cancer. The RT-PCR technique demonstrates a promising bladder tumor detection method with high sensitivity and high specificity.