半胱氨酸蛋白酶-8在膀胱肿瘤的表达及其意义

Expression and Significance of Caspase-8 in Bladder Neoplasm

目的:探讨半胱氨酸蛋白酶-8(Caspase-8)在膀胱癌发生、发展中的作用及其意义。方法:应用免疫组化Elivision法检测Caspase-8在正常膀胱组织、腺性膀胱炎、膀胱癌旁组织、膀胱移行细胞癌及膀胱腺癌中的表达;用图像分析系统测量Caspase-8的平均光密度(OD)值。结果:Caspase-8在上述组织中的平均OD值依次为(0.248±0.044)、(0.150±0.051)、(0.234±0.053)、(0.108±0.053)、(0.105±0.062)。膀胱癌中Caspase-8的表达明显低于正常膀胱组(P<0.01),Caspase-8在膀胱移行细胞癌中的表达随病理分级上升而减弱(P<0.01),且与分级存在明显的相关性(r=-0.674;P<0.01);但与病理分期无关。结论:Caspase-8在膀胱癌中表达的下调,揭示膀胱癌的演进过程与Caspase-8有密切关系。

Objective：To study the role of Caspase-8 in the development of carcinoma of bladder, as well as its clinical significance. Methods： The expression of Caspase-8 were evaluated immunohistochemically in the normal bladder samples,cystitis glandularis, paraneoplastic samples and carcinoma of bladder by measureing the average optical density with image analysis system. Results： The average optical density of Caspase-8 in normal bladder samples,cystitis glandularis, paraneoplastic samples, transitional cell carcinoma of bladder and adenocarcinoma of bladder was respectively （0. 248±0. 044）,（0. 150±0. 051）,（0,234±0. 053）,（0. 108±0. 053）,（0. 105±0. 062）. Successively the expression of Caspase 8 in carcinoma of bladder was significantly lower than that in normal bladder samples （ P〈0.01）. The intensity of Caspase-8 expression was decreased with the increasing of grades of tumor （ P 〈0. 01）, and obviously correlated with the grades （ γ=-0. 674; P〈0.01）, but incorrelated with stages of tumor. Conclusions：The down regulation of Caspase-8 expression in bladder neoplasm reveals the close relationship between Caspase-8 and the occurrence and progression of the cancer.