口蹄疫病毒特异性抗体间接ELISA检测方法的建立

Establishment of an Indirect ELISA Method for Detecting Specific Antibodies Against Foot-and-mouth Disease Virus with Recombinant Protein as Antigen

以Chap10-VP1融合蛋白为包被抗原,建立了检测口蹄疫病毒特异性抗体的间接ELISA方法。抗原包被浓度为50μg/L时,血清最佳稀释度为1∶400,病毒与阳性血清的反应可被Chap10-VP1蛋白阻断,检测的灵敏度为1∶1600。

Using Chap10 VP1 fusion protein which was constructed and expressed in our lab as coating antigen to replace FMDV.an indirect ELISA method was established by detecting the anti-VP1 antibodies in the serum from Guinea pig immunized with the inactivated vaccine of type O of FMDV. For the indirect ELISA method.the optimal concentration of coating antigen was 50 mg/L with the optimal serum dilution of 1 ： 400. In this condition.the ,specific anti-VP1 antibodies eouId be detected. The Chap10-VP1 fusion protein could block the response between FMDV and positive serum. The sensitivity of the indirect ELISA was 1 ： 1 600.