摘要
对来自青海湖的鲁道夫对盲囊线虫(Contracaecum rudolphii)核糖体DNA第一、第二内转录间隔区(ITS-1、ITS-2)进行PCR扩增、DNA单链构象多态性(SSCP)分析及序列分析,并与来自欧洲的2个姊妹种鲁道夫对盲囊线虫进行了比较。结果显示,我国青海湖的鲁道夫对盲囊线虫与来自意大利的C.rudolphii B具有一样的SSCP带型及ITS序列,但不同于C.rudolphii A,因此属于C.rudolphii B。本试验证实了ITS片段可作为遗传标记用于鉴别鲁道夫对盲囊线虫的姊妹种,从而为鲁道夫对盲囊线虫的进一步研究奠定了基础。
The aim of the present study was to identify specimens representing Contrancaecum rudolphii which were isolated from Qinghai Lake,China. The first and the second internal transcribed spacers（ITS-1 ,ITS-2） of ribosomal DNA were amplified by PCR from individual Chinese C. rudolphii and the amplicons were analysed by single-strand conformation polymorphism （SSCP）. The results of SSCP analyses revealed that all the Chinese C. rudolphii specimens represented C. rudophii B. One specimen was sequenced and the ITS-1 and ITS-2 sequences were identical to that of C. rudophii B,consistent with the results of SSCP analysis. For the first time in China,this study demonstrated that ITS-1 and ITS-2 sequences could be applied as genetic markers for the identification of Chinese C. rudolphii,and the findings have implications for studying the systematics and molecular genetics of anisakid nematodes.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2006年第1期35-38,共4页
Chinese Journal of Veterinary Science
基金
国家杰出青年科学基金资助项目(30225033)
