小麦耐逆基因-TaLEA_2转化拟南芥的研究

Transformation of a Stress-Tolerant Gene in Wheat, TaLEA_2,into Arabidopsis thaliana

研究小麦第3组LEA基因中T aLEA2对耐旱和耐盐性能的影响.将小麦第3组LEA基因T aLEA2连接在双元表达载体pB I121 C aM V 35S启动子下游,构建了能在植物中高效表达的载体pB I121-T aLEA2.通过农杆菌介导的真空渗透法,将其转入野生拟南芥中,经抗性筛选及PCR验证,获得T0代转基因植株,并用不同浓度的PEG 4000和N aC l对转基因拟南芥的耐逆性进行检测.结果表明,这些转基因植株可明显改进拟南芥在10%PEG及0.8%N aC l培养基上的生长状态.在实验条件下,转基因拟南芥的耐旱性及耐盐性均有所提高,提示T aLEA2基因在植物水分调节方面有重要作用.

This study was aimed at studying the drought- and salt-tolerant effects of TaLEA2 belonging to the third group of LEA genes in wheat. Its method was to combine TaLEA2belonging to the third group of LEA genes in wheat onto the downstream of CaMV35S promoter of double-element expression vector pBI121 and thus pBI121-TaLEA2,a vector that was capable of efficiently expressing itself in plants,was constructed. The vector was transformed into Arabidopsis thaliana by Agrobacterium-mediated depressor permeating method and then transgenic Arabidopsis thaliana plants in To were obtained by resistance screening and PCR identification;the stress tolerances of the transgenic Arabidopsis thaliana plants were tested with PEG4000 and NaCl of different concentrations;The test results showed that these transgenic plants presented a remarkably-improved growth on the medium containing 10% PEG4000 and 0.8% NaCl. The conclusion of this study is that under the experimental circumstances,transgenic Arabidopsis thaliana plants get their drought and salt tolerances improved to some extent and TaLEA2 plays an important role in regulating plant water.