肺癌及癌前病变组织芯片中PTEN的表达及其意义

Expression of PTEN and its role in lung cancerous and precancerous lesions

目的检测肺癌组织中PTEN蛋白的表达,并探讨其在肺癌发生、发展中的作用和意义。方法利用组织芯片技术和免疫组化方法,检测原发性肺癌89例、淋巴结转移性肺癌12例、癌前病变12例和正常肺标本10例中PTEN蛋白的表达情况。结果原发性肺癌中PTEN阳性率为44.9%,显著低于正常组(90.0%)(P<0.05);PTEN的表达与分化程度、淋巴结转移和临床分期相关(P<0.05)。结论PTEN蛋白在肺癌中的表达阳性率降低,并且与病情进展程度相关,PTEN的失活促进了肿瘤的发生和发展,与预后不良相关。

Objective：To investigate the role of phosphatase and tensin homologue deleted on chromosome ten （PTEN） expression in genesis and progress of lung cancer. Methods： The expression of PTEN was detected by immunohischemistry and tissue microarray technology. The total samples included 89 cases of primary lung cancer, 12 cases of metastatic cancer from lymph node, 10 cases of precancerous lesion, and 10 cases of normal lung tissue. Results： The positive rate of PTEN in primary lung cancer was 44.9%, which was significantly lower than that of normal lung tissue（90. 0%）（ P 〈0. 05）. The expression of PTEN was associated with the differentiation degrees, lymph node metastasis, and clinical stages of lung cancer （ P 〈0.05）. Conclusion：The positive rate of PTEN expression in lung cancer was down-regulated and it was related to the progress of lung cancer. PTEN gene inactivation may promote lung cancer genesis and development which may contribute to poor prognosis.