灯盏花遗传转化中组织培养受体系统的研究

Study on Tissue Culture System of Erigeron Breviscapus(Vant)Hand-Mazz.for Genetic Transformation

对灯盏花遗传转化中组织培养受体系统进行了研究,结果表明:使用MS+BA 0.2(mg/L,单位下同)+NAA 2.0的培养基,播种10 d的幼苗,0.5 cm×1.5 cm大小的真叶作为外植体,能在2周内形成愈伤组织;使用MS+BA0.5+NAA0.5培养基,附加30 g/L蔗糖,以真叶为外植体,能在2周内诱导不定芽形成;使用75μg/ml的氯霉素(Chl.)作为土壤农杆菌转化培养的选择压力,使用50μg/ml的庆大霉素(Gent.)作为转基因植株的选择压力,使用300μg/ml的头孢霉素(Cef.)作为土壤农杆菌转基因的抑菌抗生素,对灯盏花遗传转化效果最佳。

Abstract The callus can form in two weeks quickly on the MS as basic medium, BA 0.2 mg/L ＋ NAb. 2.0 mg/L as hormone and the young sprout sowing for 10 days, and true leaf of size in 0.5 cm× 1.5 cm. The adventitious bud from the true leaf can quickly form on MS ＋ BA 0.5 mg/L ＋ NAb. 0.5 mg/L as tredium,adding 30 g/L cane sugars. 75 μg/ml Chl. was used as the choice pressure of Agrobacterium tumerfaciens ,μg/ml Gent. was used as the choice pressure of turned gene plant, 300μg/mll Cef. was used as antibiotic of repressing germ and the tesuh of genetic transformation on Erigeron Breviscapus （Vant） Hand-Mazz. was the best.