摘要
用特异性引物扩增羊传染性脓疱病毒42K囊膜蛋白基因,与不同的表达载体重组后用DIG标记的特异性DNA探针进行Dot-blotting和Southern blotting杂交检测,挑选出能稳定传代的重组菌9株。经IPTG诱导后,用光敏生物素标记抗囊膜蛋白I gG进行Western blotting分析,检测到能高水平表达的重组菌pGEL-4A,每升培养液中所表达的病毒囊膜蛋白达560 mg。
42K envelope protein gene of Contagious Ecthyma virus were amplified by specification primers and recombinant to different expression vector. The 9 strains stable recombinant were screened by dot blotting and southern blotting with DNA probe labeled DIG. The high expression recombinant pGRL-4A was tested by western blotting with IgG labeled photobiotin after IPTG inducing. The results showed that the recombinant pGRL-4A produce 560 mg envelope protein in per liter medium.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2006年第1期29-32,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
甘肃省畜牧厅科研基金资助项目(项目号:GMK9006)
关键词
Off病毒
囊膜蛋白
克隆
表达
contagious Ecthyma
envelope protein
cloning
expression
