黄山药愈伤组织的诱导与分化

Induction and differentiation of callus in Dioscorea panthaica

以黄山药的叶片、茎段和叶柄作外植体，以MS为基本培养基，试验了不同激素组合对愈伤组织诱导的影响，采用正交试验法研究了愈伤组织的分化效果。结果表明，以MS％2，4-D 1～2mg／L＋6-BA 2mg／L培养基对叶片的愈伤组织诱导效果最好，接种12d后初见愈伤组织，20d后可形成大量的愈伤组织，而茎段和叶柄的诱导效果较差。分化试验结果表明，生根率最高可达85．3％，而出芽率最高仅达29．6％。

The leaf, stem segment and leaf stalk of the Dioscorea panthaica were used as explants to induce callus in different culture media. The results suggested that the better medium for inducing leave callus was MS ＋2,4-D 1-2 mg/L＋BA 2 mg/L,the callus formation was initially found after inoculating 12 days and there were much more in 20th day. They could keep vigorous growth constantly in subculture. The callus induction in stem segment and leaf stalk were inferior to that in leaf. In differentiation experiments, the highest rooting rate could reach 85.3%,but only 29.6% to bud production.