雄激素对家兔动脉粥样硬化的影响

Effect of testosterone on atherosclerosis in rabbits

目的:探讨雄激素在兔动脉硬化模型中是否具有抗动脉硬化的作用。方法:37只哈尔滨大耳白兔,饲以高脂饮食,作如下分组:去势组:切除双侧睾丸;睾酮Ⅰ组:切除双侧睾丸并给予外源性雄激素0.25mg·kg-1·d-1;睾酮Ⅱ组:切除双侧睾丸并给予外源性雄激素2.5mg·kg-1·d-1;睾酮Ⅲ组:切除双侧睾丸并给予外源性雄激素12.5mg·kg-1·d-1;假手术组。3个月后检测血中睾酮含量、血脂(包括TG、LDL-C、HDL-C)浓度、PAI活性、一氧化氮(NO)、内皮素(ET)、血管紧张素Ⅱ(AngⅡ)含量。结果:去势组血清睾酮含量明显低于其它组;TG、LDL-C同其它组相比无显著差异,而HDL-C明显降低;NO2-/NO3-含量低于其它组,而PAI活性、ET、AngⅡ却高于其它组。结论:在高脂饮食诱发的兔动脉硬化模型中,雄激素可以发挥一定抗动脉硬化的作用。

AIM： To investigate the action of testosterone on atherosclerosis in a rabbit model. METHODS： 37 male cholesterol - fed rabbits were divided into five groups： castration group： castrated rabbits without exogenous testosterone administration; testosterone Ⅰ group： castrated rabbits with exogenous testosterone 0.25 mg· kg^- 1· d ^- 1 ; testosterone Ⅱ group： castrated rabbits with exogenous testosterone 2.5 mg· kg^-1 · d^-1; testosterone m group castrated rabbits with exogenous testosterone 12.5 mg· kg^- 1· d^- 1. The sham operation group was also set. Three months later, the levels of testosterone, blood lipids （ including TG, HDL - C, LDL- C）, PAl activity, nitric oxide （NO） content, endothelin （ET）, angiotensin Ⅱ （AngⅡ） in blood were detected. RESULTS： It showed that testosterone in castration group was the lowest. There was no significant difference of TG or LDL- C between castration group and the other four groups. HDL- C in castration group was lower than that in other four groups. NO content of castration group was lower than that in others, but PAl activity, ET and Ang Ⅱ concentration were higher than that in other groups. CONCLUSION： Testosterone is a protective factor against atherosclerosis in male rabbits.