肺癌原代细胞和细胞系A549对肿瘤浸润性淋巴细胞体外增殖的抑制作用

Inhibition of lung cancer cells of primary culture and A549 cell line on the proliferation of tumor-infiltrating lymphocytes in vitro

目的研究肺癌原代细胞和细胞系A549对肿瘤浸润性淋巴细胞的抑制作用及其临床意义。方法分离和培养肺癌细胞及TIL细胞,用MTT法检测原代肺腺癌细胞及腺癌细胞株A549对TIL体外增殖的抑制作用及FasL抗体对TIL的保护作用。结果原代肺癌细胞及A549细胞株对TIL均有不同程度的抑制作用。结论肺癌细胞对TIL细胞的抑制作用可能是机体逃避免疫攻击的机制之一。

Objective To study the inhibition of lung the proliferation of tumor-infiltrating lymphocytes （TIL） in cancer cells of primary culture and A549 cell line on vitro and its clinical implications. Methods Human lung cancer cells and TILs were isolated from 8 cases of primary lung cancer and cultured. The inhibition of isolated human cancer cells and A549 cells to TILs was detected by MTr method. Results Lung cancer cells of primary culture and A549 cells can kill TILs to various degrees. Conclusion Inhibition of lung cancer cells to TIL may be one of the mechanisms of immune escapes.