原发性肝癌不同病理组织类型中肝干细胞的起源分析

Origin of hepatic stem cells in human hepatocellular carcinoma

目的观察原发性肝癌不同病理组织类型中肝干细胞的活化、分布、来源和免疫表达特征。方法应用HE染色、免疫组化S-P方法观察94例肝细胞癌(HCC)和12例肝内胆管细胞癌(ICC)和10例混合型肝癌的肝干细胞情况,用5例肝硬化和4例正常肝组织为对照。结果在原发性肝癌不同病理组织类型中干细胞免疫标志均有不同程度的表达,均可观察到肝干细胞向肝癌细胞的转化,以混合型肝细胞癌中肝干细胞免疫表型表达率最高(P<0·05)。结论不同病理组织类型的肝癌组织中均存在不同分化状态和不同来源的肝干细胞,可能由于肝干细胞(hepaticstemcell,HSC)分化不全或分化异常所致。

Objective To investigate the activation, distribution, origin, and expression of hepatic stem cells （HSC） in different histological types of primary liver carcinomas. Methods The histological and immunohistochemical features of 94 cases of hepatocellular carcinoma （HCC） , 12 cases of intrahepatic cholangiocarcinoma （ICC） and 10 cases of mixed hepatocarcinoma were examined by HE staining and immunohistochemistry SP method, with 5 cases of sclerotic liver and 4 cases of normal liver tissues as control. Results HSC expression was observed and the transformation from HSC to carcinoma cell was also noted in the liver. CK7, CK19, c-kit, Thy-1, and AFP were found expressed in different types of hepatic carcinomas and the greatest intensive expression was found in the mixed hepatocarcinoma （ P 〈 0. 05 ）. Conclusion There exist varied differentiations and origins of HSCs in different histological types of primary hepatic carcinoma tissues, which is presumably due to poor or abnormal differentiation of HSCs.