摘要
目的研究德氮吡格(TNBG)对人肝癌细胞株QGY-7701基因表达的影响,探讨其抗肿瘤作用的分子机制。方法采用基因芯片检测德氮吡格处理人肝癌细胞株QGY-7701前后mRNA的改变情况。结果基因芯片筛选出差异表达基因共1200条,尤以脂代谢相关基因变化最显著:脂肪合成有关的10条基因表达上调,脂肪分解有关的3条基因和脂肪转移有关的1条基因表达下调。结论德氮吡格的抗肿瘤效应可能是通过干扰细胞的脂代谢来实现的。
Objective To study the effects of Tet.razanbigen （TNBG） on the gene expression of human hepatocellular carcinoma cell line QGY-7701 for elucidating its antitumor mechanism. Methods The cDNA microarray technique was used to explore the gene expression profiles of the tumor cells before and after TNBG treatment. Results The cDNA microarray screened 1 200 differential expression genes. The expression difference of genes involving lipid metabolism was most remarkable, in which 10 genes involving lipid biosynthesis were up-regulated, 3 genes responsible for lipid degradation and 1 for lipid transport were down-regulated. Conclusion The mechanism of antitumor effect of TNBG is probably via interfering with lipid metabolism of tumor cells.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2006年第2期151-153,共3页
Journal of Third Military Medical University
基金
国家自然科学基金资助项目(30371632)
重庆市科委攻关课题(2002-14-25)
重庆市教委课题(2002-18-04)~~
关键词
德氮吡格
肝肿瘤
基因表达
脂代谢
tetrazanbigen
hepatocellular carcinoma
gene expression
lipid metabolism
