摘要
目的:观察人神经生长因子(hNGF)对早期糖尿病多发性神经病(DPN)大鼠坐骨神经中calpainⅡ表达的影响。方法:用链佐星(STZ)一次性腹腔注射诱导糖尿病大鼠模型,在DPN4周(造模后4周)腹腔注射hNGF90U/(kg·d),共4周。用原位杂交和免疫组化方法观察坐骨神经calpainⅡmRNA和蛋白表达。结果:DPN8周大鼠坐骨神经calpainⅡmRNA和蛋白表达明显减少。用hNGF治疗的DPN大鼠calpainⅡmRNA和蛋白表达增加。结论:hNGF治疗能纠正早期DPN大鼠坐骨神经calpainⅡmRNA和蛋白表达异常。
Aim：To observe the effect of human nerve gyowth factor（hNGF） on Calpain Ⅱ expression in sciatic nerves of experimental diabetic polyneuropathy rats. Methods： Diabetic rat was induced by a single intraperitoneally injection of streptozocin. After 4 weeks of diabetes, hNGF 90 U/（ kg·d ） was injected intraperitoneally for 4 weeks. The level of calpain Ⅱ mRNA and protein expressions in sciatic nerves were measured by in situ hybridization and immunohistochemistry techniques. Results：After 8 weeks of diabetes, both calpain Ⅱ mRNA and calpain Ⅱ protein expressions were decreased significantly. Treatment with hNGF for 4 weeks, calpain Ⅱ mRNA and protein expressions were increased. Conclusion： Administration of hNGF can partly reverse abnormal expression of calpain Ⅱ in sciatic nerves of experimental diabotic polyneuropathy rats.
出处
《中国临床神经科学》
2006年第1期20-24,共5页
Chinese Journal of Clinical Neurosciences
