叶酸缺乏对孕鼠子代心脏中NKx2.5基因表达的影响

Effects of Folate Deficiency during Pregnancy on Expression of NKx2.5 Gene in Heart of Offspring

目的探讨叶酸缺乏孕鼠子代心脏发育过程中NKx2.5基因和蛋白表达改变。方法成熟雌性36只SD大鼠随机分为实验组和对照组各18只,分别喂以缺乏叶酸和添加叶酸的纯合饲料。2周后与成熟SD雄性大鼠交配,分别取孕13.5 d、孕17.5 d胚胎鼠及新生鼠心脏。用RT-PCR检测NKx2.5基因mRNA表达。Western-blotting测GATA-4蛋白表达水平。结果NKx2.5基因mRNA及其蛋白在孕13.5 d、孕17.5 d胚胎心脏及新生鼠心脏中的表达量,实验组均显著低于对照组(P均<0.05)。结论叶酸缺乏影响NKx2.5基因和蛋白表达水平,可能导致心脏发生发育中形态改变,从而造成心脏功能缺陷。

Objective To study the expression of NKx2.5 on the heart of offspring during the development of embryo, whose mother is deficient of folic acid. Methods 1. Control group involving 18 rats and study group involving 18 rats were chosen from the total 36 adult female SD rats randomly copulate with the male normal rats after feeding different fodder for 2 weeks. The heart of the 13.5 days, 17.5 days embryos and the newborns were obtained; 2. the expression of NKx2.5mRNA by RT- PCR was observed; 3. the expression of NKx2.5 protein by Western - blotting was investigated. Results 1. The expression of NKx2.5 mR.NA of study group was weaker than control group in heart of the 13.5 days, 17.5 days embryos and the newborns（P〈0.05） ;2. In the heart of the 13.5 days,17.5 days embryos and the newborns, the expression of NKx2.5 protein of study group was weaker than that of control group（ P 〈 0.05）. Conelesion The change of exprssion of NKx2.5 mRNA and protein might be resulted from folic acid deficiency during the pregnancy, which might lead to the malformation of the heart.